A continuacion preseto el abstract de un muy bue articulo de Peter Setlow  una persona que ha estudiado por largo tiempo a las esporas de Bacillus Subtillis

Abstract

Log phase Bacillus subtilis cells lacking the

mscL

large conductance are sensitive to an osmotic downshock

gene encoding the mechanosensitive (MS) channel of

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downshock resistance in late log and early stationary phase

growth that is partially dependent on three likely MS channel

proteins of small conductance (MscS), YfkC, YhdY,

and YkuT.

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the MscL-, YfkC-, and YkuT-GFP fusions were functional

and overexpression of YkuT-GFP, or YkuT alone abolished

log phase

Western blot analysis found high levels of MscL-GFP in

early exponential phase cells with levels subsequently

decreasing greatly. MscS-GFP proteins were present in

exponential phase cells, but again disappeared almost completely

in stationary phase cells and these proteins were not

detected in spores. Western blot analyses further showed

that MS-GFP proteins were associated with the plasma

membrane, as expected. Fluorescence microscopy con-

0.5 M. However, B. subtilis mscL cells develop osmoticBacillus subtilis MS proteins were fused withXuorescent protein (GFP) at their C termini; at leastmscL cells’ osmotic downshock sensitivity.

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plasma membrane, with non-uniform distribution of these

proteins along this membrane consistent with but by normed the localization of MscL-GFP and YhdY-GFP to the